| abstract |
This invention is related to novel probes, probe sets, methods and kits pertaining to the detection of bacteria of the Salmonella genus. The probes, probe sets, methods and kits of this invention are particularly useful for the detection, identification and/or enumeration of bacteria of the Salmonella genus. It is an advantage of the identified probes that they do not substantially cross react with bacteria of the closely related Citrobacter genus. Preferably, the probes of this invention are prepared as PNA probes and most preferably the probing nucleobase sequence comprises a segment that is at least ninety percent homologous to the nucleobase sequence; GTG-TTA-AAG-TGA-ACC (Seq. Id. No. 1), AGC-CTT-GAT-TTT-CCG (Seq. Id. No. 2) or ACC-TAC-GTG-TCA-GCG (Seq. Id. No. 3). Also disclosed is a particularly useful method for specifically detecting, identifying and/or quantitating organisms of a genus or species when the probes chosen possess overlap with certain organisms that are likely to contaminate the sample and otherwise lead to false positive results. The probes, probe sets, methods and kits of this invention are particularly well suited for use in ISH or FISH assays including assays of the multiplex format. |