http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002051983-A1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6865 |
filingDate | 2001-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c979562c2ac2a114abdebe2fcf69743c |
publicationDate | 2002-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2002051983-A1 |
titleOfInvention | Detection of amplified products in nucleic acid assays following nuclease treatment |
abstract | The present invention provides a method for detecting nucleic acid amplification product of a target-dependent nucleic acid amplification process involving one or more probes or primers, comprising the steps of: a) treating said product with a nuclease reagent whereby said product is substantially hydrolysed into its mononucleotide components; b) detecting said mononucleotide components. The amplification product is suitably selected for by use of a nuclease reagent that is specific for polyribonucleotide when said product is a polyribonucleotide, and is specific for polydeoxyribonucleotide when said product is a polydeoxyribonucleotide. Alternatively, the detection step may be specific for the mononucleotide components from the product by being selective between ribonucleotides and deoxyribonucleotides. |
priorityDate | 1998-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1698.