http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002048806-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-525 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2720-10021 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate | 2001-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9b79254965f30a2048a5d910e8cccbb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0300f2cc7f303fffd93a0ffe636d64e3 |
| publicationDate | 2002-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2002048806-A1 |
| titleOfInvention | Poultry virus isolates and method |
| abstract | In accordance with the present invention, numerous experiments were conducted to determine the role of infectious bursal disease (IBD) virus in the induction of lesions associated with proventriculitis syndrome in chickens. Parameters examined included age of the chicken at IBD virus exposure, concentration of IBD virus at exposure, the strain of IBD virus, dietary influence in the presence of IBD virus, mixed IBD virus infection, auto-immune mediated IBD reactions associated with lesion production, viral induced apoptotic tissue injury and isolation and characterization of the causative agent. The experiments were carried out in SPF white leghorns, with the experimental birds being examined for the presence of gross and microscopic lesion at 4 and 11 days post challenge. Tissue homogenates were analyzed for the presence of IBDV at 4 and 11 days post challenge with Antigen Capture ELISA (AC-ELISA). Determinations of neutralizing antibody levels and IgG antibody responses were monitored as well as depletion of serum complement following IBDV infection. Physical parameters were also considered utilizing body weights and organ to body weight ratios to determine IBDV effects following experimental challenge. Physical parameters indicated that the primary viral response is predominantly in the bursa of Fabricius, but changes were also noted in the proventriculus. Physical changes in the proventriculus occurred primarily during the acute stage of the IBD virus infection. Gross and microscopic lesions in the proventriculus were exacerbated by the presence of copper sulfate supplementation in the feed. There were also very evident negative effects on the weight gain of SPF white leghorn chickens given copper sulfate feed supplementation. Infectious bursal disease virus strains show different affinities for producing lesions in the proventriculus following challenge. Standard challenge IBD virus USDA/STC stain produced the most consistent lesions in the proventriculus often accompanied by hemorrhage. This correlates well with antibody enhanced pathology as observed with antigen-antibody complex activation of serum complement. Evidence of yet another mechanism of IBDV induced pathology, was demonstrated through the use of TUNEL apoptosis staining of infected tissues. The degree of apoptotic involvement was also reflected in the pathogenicity of the IBD virus strain used for challenge. Infectious bursal disease viral inclusions were demonstrated by thin section electron microscopy in the proventriculus at 4 days post challenge. Viral inclusions resemble previously documented inclusions produced in the bursa following IBD infection. In accordance with the present invention, infectious bursal disease viruses were isolated from broiler chickens experiencing proventriculitis in, for example, Oklahoma, Texas, West Virginia, and California. Proventriculitis is a major problem to the broiler industry. Chicken virus isolates Texas RB 3, Texas RB 4, HBS, F57-7, W/L 39, GAR 1, and the like have been isolated. These viruses may be one of the major causes of this condition and if this proves to be true, could be utilized as or in a vaccine to prevent the disease condition. The viruses could be a significant finding in the search for a causative agent for proventriculitis in broiler chickens and as such may be utilized in the development of a vaccine or vaccines. The viruses can be attenuated to be used as a modified live vaccine or utilized in an inactivated form in a killed vaccine. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102641366-A |
| priorityDate | 1998-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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