| abstract |
The invention describes a method and its use for producing complex multienzymatical, storage resistant reaction mixtures for synthesizing, modifying or analyzing polypeptides and optionally nucleic acids characterized in that native or artificial enzymatical, active protein mixtures with reaction buffers, cofactors and substrates are prepared so that they are ready for use and are storage resistant such that only user-specific key components (e.g. mRNS) are missing to start the desired enzymatical reaction(s). In the method a stabilizer is added to the reaction mixtures in the solution which, on the one hand, increases the reacting capacity of the multienzymatical systems and, on the other hand, protects the unstable reaction components from losing their biological activity or their biologically active structure while being made storage resistant and during storage. The reaction mixture is made storage resistant by being easily freeze dried under a vacuum and then durably stored at 4-10° C. (refrigerator temperature). Before use the user has to simply reconstitute the ready prepared reaction mixture by adding the original volume of H 2 O and start the desired enzymatical reaction(s) by adding the user-specific component(s). |