| abstract |
Currently, three technologies are utilized for analysis of gene expression: hybridization-based technologies, PCR-based technologies, and sequence-based technologies. The present invention provides a method for analyzing the presence and/or amount of a specific nucleic acid using a solid support and a capture probe complementary to a region of a target nucleic acid, and polymerizing a labeled extension complementary to the target nucleic acid. The invention provides a method of analysis of all types of nucleic acids, and can be used to study multiple genes in a single assay using different capture probes conjugated to different class of microspheres that can be mixed in any desired combination. |