http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002031808-A1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-0003 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 |
filingDate | 2001-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c4be666e0a0586233b098f1fb3ec663 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d0736663f27c648af0bb976ae1b7f00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8cabfbbe420278293dcb757f2cc5ce6f |
publicationDate | 2002-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2002031808-A1 |
titleOfInvention | Whole cell and cell-debris polysaccharide |
abstract | The present invention provides for a method for obtaining a polysaccharide substantially free from whole bacterial cells and bacterial cell debris including: n (a) fermenting a Sphingomonas bacterium, which produces the polysaccharide, to produce an aqueous fermentation broth having the polysaccharide dissolved therein; n (b) diluting the fermentation broth with an equal volume of deionized water; n (c) partially hydrolyzing the polysaccharide by exposing the fermentation broth resulting from step (b) to a temperature in excess of 100° C. for a time period from about ten minutes to about one hour; n (d) removing bacterial cells from the polysaccharide by centrifugation of the fermentation broth and recovery of a supernatant aqueous liquid; n (e) precipitating the polysaccharide from the supernatant aqueous liquid by adding a second liquid to the supernatant aqueous liquid, wherein the second liquid is miscible with water, non-reactive with the polysaccharide, and in which the polysaccharide is substantially insoluble; and n (f) harvesting the polysaccharide resulting from step (e) by separating it from the supernatant aqueous liquid. |
priorityDate | 2000-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.