| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-13043 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
1997-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_319a350bb7a486cd4d28a7bdda5b0155
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_658d9f5df0fe850b838c814c82bd1607
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_365c8431cec00888ef5385bee0b56033
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_546856bc7b8f1eaab76712853017149c |
| publicationDate |
2002-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2002015979-A1 |
| titleOfInvention |
Vehicles for stable transfer of green fluorescent protein gene and methods of use for same |
| abstract |
The present invention describes an efficient retroviral or viral based method that allows easy and quick identification of gene transfer in living, transduced mammalian cells. Retroviral and viral vector producer cells were generated containing a gene for an improved humanized red-shifted, Green Fluorescent Protein (hRGFP) which increases the resulting fluorescence yield after excitation. This humanized, red-shifted GFP (hRGFP) gene was cloned into several vectors and transfected into various packaging cell lines to produce vibrant green fluorescence after excitation with blue light at 450-490 nm. These vectors represent a substantial advance over currently available gene transfer marking systems or wild-type GFP marker systems none of which have been stably transfected into cells. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2003285009-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-4654033-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006506984-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1579004-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1579004-A2 |
| priorityDate |
1996-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |