http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002009799-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2000-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2e7d88c3ee3d6156702575ecca10d80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ea241f09b530232b0c1b75ba46da45f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b7d2214c30b13c7cbd9d47a39c8310e |
| publicationDate | 2002-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2002009799-A1 |
| titleOfInvention | Isolation and purification of nucleic acids |
| abstract | In one aspect the present invention provides methods for isolating nucleic acid molecules from a cell, the methods comprising (a) contacting a cell with a solution comprising a biopolymer-degrading enzyme, provided that the biopolymer-degrading enzyme is not a nuclease, and (b) contacting the cell with a solution comprising a hydrophobic surfactant to yield a cell suspension comprising cell, biopolymer-degrading enzyme and hydrophobic surfactant, wherein the hydrophobic surfactant has a critical micelle concentration less than 3.0 mM and the concentration of the hydrophobic surfactant in the cell suspension is at least 0.05% (v/v). In another aspect the present invention provides isolated DNA preparations comprising at least 80% supercoiled DNA. In another aspect the present invention provides isolated nucleic acid preparations having an A 260/230 ratio of at least 2.0. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013260407-A1 |
| priorityDate | 1999-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 158.