http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2001053518-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 1999-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5089e9cc0ab119c89c21218a1159c8f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d47ff465a80ba6341103e38822027634 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6dc3aff30527578f97d639147195b911 |
| publicationDate | 2001-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2001053518-A1 |
| titleOfInvention | Method of assay of target nucleic acid |
| abstract | A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once and n a step of measuring a fluorescent signal in the presence of the reagent (I) at least once after addition of at least the reagents (A) to (H); n (A) a first single-stranded oligonucleic acid complementary to a sequence neighboring the 5′ end of the specific nucleic acids sequence in the single-stranded RNA, n (B) a second single-stranded oligo DNA complementary to a 3′-end sequence within the specific nucleic acids sequence, n (C) an RNA-dependent DNA polymerase, n (D) deoxyribonucleoside triphosphates, n (E) a third single-stranded oligo DNA having (1) a promoter sequence for a DNA-dependent RNA polymerase, (2) an enhancer sequence for the promoter and (3) a 5′-end sequence within the specific nucleic acids sequence, in this order from the 5′ end, n (F) a DNA-dependent DNA polymerase, n (G) a DNA-dependent RNA polymerase, n (H) ribonucleoside triphosphates, and n (I) a fourth single-stranded oligo DNA complementary to the specific nucleic acids sequence which is labeled so that it gives off a measurable fluorescent signal on hybridization with a nucleic acid containing the specific nucleic acids sequence. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6841349-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02090563-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005037361-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010304377-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106715724-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8785126-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022065413-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008213859-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6780588-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020203896-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7211385-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011143352-A1 |
| priorityDate | 1998-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 158.