| abstract |
The present invention relates to variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: n R490A,L,I,Q,M,E,D; n A399G; n F397N,Y,E,Q; n P395A,S; n R360A,L,I,Q,M,E,D; n K353Q,M; n S402A,T,V,S; n D292L; n A501S; n W350F,Y; n V495A,T,V,S; n K394 A,L,I,Q,M,E,D; n wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1. |