| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_916075766043ce900399a4c2eee9f054 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate |
1997-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89b96453c6606e8d5545f83e961ec7d1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_610b7337d7532ee7550c3c7f6f6767ad
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b222648285f437f7bce12a7b74cbe83 |
| publicationDate |
2001-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2001012613-A1 |
| titleOfInvention |
Thermostable polymerases having altered fidelity and method of identifying and using same |
| abstract |
The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011157436-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103509767-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9765312-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115161301-A
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014509193-A
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013534817-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013531985-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9879237-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013530694-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017191044-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9353403-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9896670-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10023850-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10544404-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105039278-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9902944-B2 |
| priorityDate |
1996-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |