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filingDate 2019-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0bc46422cdd2f7488c6fd7e2fe38bc84
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publicationDate 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-11536717-B2
titleOfInvention Method of agglutination immunoassay
abstract The present invention provides a particle enhanced agglutination immunoassay including the steps of: mixing a sample solution containing an analyte with a solution containing insoluble carrier particles carrying a binding partner or binding partners for the analyte to prepare a mixed solution; determining a variation (i) in intensity of light scattered from the mixed solution based on a difference in intensity of scattered light between first and second time points; determining a variation (ii) in absorbance of the mixed solution based on a difference in absorbance between third and fourth time points; and correlating the determined variation (i) in intensity of scattered light and the determined variation (ii) in absorbance with an amount of the analyte present in the sample using a calibration curve plotted based on the variation in intensity of scattered light and a calibration curve plotted based on the variation in absorbance. The present invention employs measurements of the intensity of scattered light and the absorbance in combination for a single assay, and thus provides a particle enhanced agglutination immunoassay which achieves higher sensitivity and a wider dynamic range than conventional assays.
priorityDate 2013-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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