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filingDate 2016-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-11535674-B2
titleOfInvention Bivalent bispecific antibody hybrid protein expression and preparation methods
abstract The present disclosure discloses a method for expressing and preparing a bivalent bispecific antibody. In the present disclosure, each portion of a bivalent bispecific antibody and an immune hybrid protein thereof is respectively expressed in a suitable prokaryotic or eukaryotic cell system, separated and purified by high-performance affinity chromatography, and then spliced in vitro by trans-splicing reaction mediated by an intein, to prepare the bivalent specific antibody and an immune hybrid protein thereof.
priorityDate 2016-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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