http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11345952-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2545-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6846 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 |
| filingDate | 2017-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c32ced1041d530e5b5f0319987dd249 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04014980e809ce52a32cabe8a3addd89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9b92df72b5b39917cf137ff733453d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_698437295f259f32f31afc0fea17cb07 |
| publicationDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-11345952-B2 |
| titleOfInvention | Internal amplification control |
| abstract | PCR that allows the researchers to amplify a desired DNA requiring only tiny amounts of sample. Such amplification reactions are technically challenging and are often hampered by several practical issues such as the presence of PCR inhibitors, sample degradation and low quantities of said sample. The invention addresses these issues with a method for evaluating the amplification efficiency and/or the presence of inhibitors and/or degradation and/or performing a quantification of a nucleic acid in a real-time amplification reaction comprising: optionally amplifying in a reaction composition a first target nucleic acid using a first primer pair in a real-time amplification reaction, (i) amplifying in said reaction composition one or more second internal nucleic acid control templates (IC) with a length of between 50 and 2000 nucleotides, wherein the second nucleic acid has a sequence selected from the group of: i) SEQ ID NO. 1 or a sequence that is differs by no more than 15% therefrom, ii) a reverse complement of SEQ ID NO. 1 or a sequence that is differs by no more than 15% therefrom, iii) a sequence or a reverse complement thereof comprising the final 18 to 30 3′-nucleotides of SEQ ID NO. 1 at its terminal 3′-end and the final 18 to 30 5′-nucleotides of SEQ ID NO. 1 at its terminal 5′-end, or terminal ends that differ by no more than 5% from SEQ ID NO. 1, wherein between these terminal 3′-ends and 5′-ends the nucleic may have any nucleotide sequence and is between about 30 and about 1950 nucleotides in length. |
| priorityDate | 2016-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 108.