http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11198896-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1966f3c715acc0fa006db05d916301a7 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01201 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01201 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P33-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P33-02 |
filingDate | 2020-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9c688240a59de94b0c588d8042ca659 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41ed5187331db77e91b45dd6b94092ea |
publicationDate | 2021-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-11198896-B2 |
titleOfInvention | 7-beta-hydroxysteroid dehydrogenase mutants and process for the preparation of ursodeoxycholic acid |
abstract | One aspect of the invention provides a nucleic acid encoding a 7β-hydroxysteroid dehydrogenase (7β-HSDH) that catalyzes at least the stereospecific enzymatic reduction of a 7-ketosteroid to the corresponding 7-hydroxysteroid. The enzyme includes a mutation at position 64 of SEQ ID NO:2 or in the corresponding sequence positions of an amino acid sequence derived therefrom with at least 90% sequence identity to SEQ ID NO:2. The mutation at position 64 is the mutation R64X1, wherein X1 represents E, D, T, L, S, P, V, K, C, A, G, Q, F, W, I or Y. The enzyme shows the following property profile in comparison with the 7β-HSDH with SEQ ID NO:2: (a) an increased specific activity (Vmax [U/mg]) for NADPH in the enzymatic reduction of dehydrocholic acid (DHCA) with NADPH as cofactor. |
priorityDate | 2014-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 782.