Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bf455a20b1ecbcf7d35561fc0a9d8e94 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-22031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2015-8518 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-203 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
filingDate |
2019-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2021-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_422476fc51ab56429ea0822e3930e8df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed21ebd7e3a953543c4105c90ae06f47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f80b692787a11f21e4040b2052a7f53 |
publicationDate |
2021-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-11098310-B2 |
titleOfInvention |
Expression from transposon-based vectors and uses |
abstract |
Recombinant expression vectors are disclosed that include a control sequence for recombinant expression of proteins of interest; the control sequence combines a mCMV enhancer sequence with a rat EF-1alpha intron sequence. Some of the vectors are useful for tetracycline-inducible expression. Some of the vectors contain a 5′ PiggyBac ITR and a 3′ PiggyBac ITR to promote genomic integration into a host cell chromosome. A method of selecting a stable production cell line for manufacturing a protein of interest is also disclosed. Also disclosed are mammalian host cells comprising the inventive recombinant expression vectors and a method of producing a protein of interest, in vitro, involving the mammalian host cell. |
priorityDate |
2016-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |