http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11078476-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_93f1ee19dc8b1585c20bc59c0f68ed70 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2018-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9c07d0fa67aa04c9953a4ae55fab569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6a2485f40f3445fe58de0f4a9ab3a73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c02c9a721853b3716608f83dc5ae4bdb |
publicationDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-11078476-B2 |
titleOfInvention | Methods and kits for extracting nucleic acids from paraffin embedded samples |
abstract | This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis. |
priorityDate | 2017-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 718.