| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2198967fc1fe37568301cd993d930568
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d11bb2fb690af8a9550a4e65ba4bb4cc |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-14001 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-42 |
| filingDate |
2016-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2021-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47b3d97a0951df656543de56ce47593e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0ab7d188284811fc98d7be4a66e6b32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aaa45f05ad08c38102f35b56911651b3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12ed45b55fae7babbc7dd98782091fa2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c8bc8d9941d82fd320c292284f1e4fa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa8b72e546695444b2c332130204c4af |
| publicationDate |
2021-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10988786-B2 |
| titleOfInvention |
Mutant 4-hydroxyphenylacetate 3-hydroxylases and uses thereof |
| abstract |
The present invention relates to genetically modified enzymes obtained by rational design of the active site binding pocket of the prototypic enzyme 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) for hydroxylating a 4-hydroxyphenyl compound to yield a 3,4-dihydroxyphenyl compound and to biotechnological methods including in vivo and in vitro methods using said enzymes or catalytically active fragments thereof. Further provided is a method either using a suitable oxidase or hydroxylase further enabling the subsequent site specific methylation of the 3,4-dihydroxyphenyl compound in a coupled enzymatic reaction by providing a suitable O-methyltransferase. Finally, compositions obtainable by the aforementioned methods are disclosed. |
| priorityDate |
2015-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |