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filingDate 2017-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ee5dda56b7f18181833c9b27f4947cb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_447189f06d03d00f722ebea9e6797900
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publicationDate 2021-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10988742-B2
titleOfInvention Penicillin expandase mutants, DNA coding the mutants, reagent kit containing the mutants and the application
abstract Provided herein are penicillin expandase mutants, DNA coding the mutants, reagent kit containing the mutants and the application. The penicillin expandase mutants using SEQ ID NO.: 2 in the Sequence Listing as a reference sequence, have at least one amino acid mutation at residue positions corresponding to threonine at position 42 and glutamine at position 126, wherein, amino acid at position 42 is substituted by any other natural amino acid except threonine, amino acid at position 126 is substituted by any other natural amino acid except glutamine. The penicillin expandase mutants of present invention have increased its thermostability and catalytic activity; it is more suitable for commercial and industrial applications.
priorityDate 2016-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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