| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc2b03342511abc88f60c63f2acd96d0 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00585
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00596
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J19-0046
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-159
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-131
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1034
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-191
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J19-00 |
| filingDate |
2020-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f29aa7de39ea6846589ef217d54a7d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce1ad2fb711d381c8416af7676f9a010
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7df534e331625922ebd4d400dfc88fe7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d85fc2fab120fd396f13249d7961ded0 |
| publicationDate |
2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10981137-B2 |
| titleOfInvention |
Enrichment of DNA sequencing libraries from samples containing small amounts of target DNA |
| abstract |
Provided herein is a method for capturing DNA molecules in solution. The method may comprise: extracting DNA from a sample that comprises endogenous DNA and environmental DNA to produce extracted DNA; ligating universal adaptors to the extracted DNA; hybridizing the extracted DNA, in solution, with affinity-tagged RNA probes generated by: in vitro transcribing a library of fragmented reference genomic DNA that has been ligated to an RNA promoter adaptor, in the presence of an affinity-tagged ribonucleotide; binding the product with a capture agent that is tethered to a substrate in the presence of RNA oligonucleotides that are complementary to the adaptors, thereby capturing the hybridized DNA molecules on the substrate; washing the substrate to remove any unbound DNA molecules; and releasing the captured DNA molecules. A kit for performing the method is also provided. |
| priorityDate |
2013-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |