http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10870872-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e071d18fea054bf8d1420d483e78ed1 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-629 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-519 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate | 2017-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ab8c5dc86e841629a207eba9eaabb48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07422293c47872d65cae4f1e00782677 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ffed74e5fde3ffa1d92944e8e795bb3 |
publicationDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-10870872-B2 |
titleOfInvention | Enzymatic nucleic acid synthesis |
abstract | The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3′ terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3′ terminal nucleotide such that the selected nucleotide becomes a 3′ terminal nucleotide, and repeating the addition step until the polynucleotide is formed. |
priorityDate | 2016-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 277.