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filingDate 2014-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10865436-B2
titleOfInvention Microfluidic apparatus, method, and applications
abstract A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.
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