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filingDate 2019-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10859555-B2
titleOfInvention Methods of isolating and detecting metal ions from proteins
abstract A method of detecting a metal ion in a protein-containing sample includes adding a protein degrading enzyme to the protein-containing sample to form an enzyme degradation product, adding an acid to the enzyme degradation product to provide a mixture, filtering the mixture to provide a supernatant, extracting the supernatant with an organic solvent to remove organic solvent soluble byproducts to provide a washed aqueous layer, and detecting the metal ion in the washed aqueous layer. The method is amenable to the detection of heavy metal ions in complex products such as milk. A kit includes reagents for performing the method.
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