| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6419819278c4e69622dff8f635023155 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-115
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1062
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-04 |
| filingDate |
2018-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2020-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_256fe9daa6ddfd11bf6ecf7d848343e4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5773864f74ce80be6db8372386332e6c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2eb748d78c31e149a6ae063dc3c6f9ff |
| publicationDate |
2020-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10781442-B2 |
| titleOfInvention |
High-speed in vitro screening method |
| abstract |
The purpose of the present invention is to provide a high-speed in vitro screening method for any library selected from the group consisting of a cDNA display library and a nucleic acid aptamer library. This high-speed in vitro screening method involves: (i) a step for preparing positive spherical structures formed by immobilizing a target molecule on a spherical structure and negative spherical structures having no target molecules immobilized thereon; (ii) a step in which a target detection molecule, selected from the aforementioned library having a library size of greater than or equal to 1010, is bonded on each spherical structure to obtain spherical conjugates; (iii) a step in which the spherical conjugates are sorted into positive spherical conjugates and negative spherical conjugates with a cell sorter; (v) a step for supplying the nucleic acid on the surface of the sorted spherical conjugates for PCR; (vi) and a repetition step for repeating steps (i) to (v) above using DNA obtained by PCR. |
| priorityDate |
2016-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |