Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c04036bba1a94348a49305ea1aa341e0 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-179 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-161 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
filingDate |
2016-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2020-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb344be8943844768b159787951a2125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74a1677d84e4102599eefc90445b45f2 |
publicationDate |
2020-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-10760120-B2 |
titleOfInvention |
High multiplex PCR with molecular barcoding |
abstract |
The present disclosure provides methods and kits for performing high multiplex PCR using molecular barcodes. The methods disclosed herein separately extend a set of primers (BC primers) that each comprise a target-specific sequence, a molecular barcode and a universal sequence, and amplify the resulting extension products using another set of primers (LA primers) that each comprise another target-specific sequence and a universal sequence. The methods may further comprise amplification using universal primers (preferably comprising an adapter). |
priorityDate |
2015-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |