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inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b32c1fdc5d452f8b6d4175e437a97f45
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publicationDate 2020-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10752907-B2
titleOfInvention Promoter variants
abstract An isolated and/or artificial pG1-x promoter, which is a functional variant of the carbon source regulatable pG1 promoter of Pichia pastoris identified by SEQ ID 1, which pG1-x promoter consists of or comprises at least a part of SEQ ID 1 with a length of at least 293 bp, characterized by the following promoter regions:n a) at least one core regulatory region comprising the nucleotide sequences SEQ ID 2 and SEQ ID 3; and b) a non-core regulatory region, which is any region within the pG1-x promoter sequence other than the core regulatory region; wherein the pG1-x promoter comprises at least one mutation in any of the promoter regions and a sequence identity of at least 80% in SEQ ID 2 and SEQ ID 3, and a sequence identity of at least 50% in any region other than SEQ ID 2 or SEQ ID 3; and further wherein the pG1-x promoter is characterized by the same or an increased promoter strength and induction ratio as compared to the pG1 promoter, whereinn the promoter strength is at least 1.1-fold increased in the induced state as compared to the pG1 promoter, and/or the induction ratio is at least 1.1-fold increased as compared to the pG1 promoter.
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