http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10725022-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_803d26f802517f0bf71214db6adec52c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_134e761e8dcbff4985fa1e77a9c2062e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-502 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5073 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2017-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0bf3deff4bdf4a60c5f6297fba27464 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_daea49d928ea82f8c531068d46e04d12 |
| publicationDate | 2020-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-10725022-B2 |
| titleOfInvention | Arrays of discrete cell culture microenvironments, methods of making such arrays and uses thereof |
| abstract | A method of making an array having discrete volumes of cell culture micro-environments possessing different properties influencing the behavior of encapsulated cells, in particular proliferation, colony-formation, differentiation, migration, or combinations thereof. The method comprises a) providing different hydrogel precursor molecules, where the precursor molecules are linear or branched hydrophilic polymers for building up cell culture micro-environments; b) combining and dispensing different combinations of the hydrogel precursor molecules, according to step a), onto discrete volumes of a multi-well plate; c) adding one or more biologically active molecules to the discrete volumes and either attaching the molecules to one of the hydrogel precursor molecules present or a hydrogel, formed in step e), or allowing them to diffuse freely; d) adding cells onto/into the discrete volumes of the multi-well plate; and e) crosslinking the hydrogel precursor molecules based on an enzymatically catalyzed reaction or a Michael addition reaction to form a hydrogel matrix. |
| priorityDate | 2013-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1147.