http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10683488-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4243ece7d4d335b6539360fe7476fe91 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-35 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N63-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N63-50 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-50 |
| filingDate | 2018-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db97dec8af7547cbc5fb1ba194607bb2 |
| publicationDate | 2020-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-10683488-B2 |
| titleOfInvention | System and method for creating crystals of insect acetylcholinesterase |
| abstract | A method of creating crystals of insect acetylcholinesterase. A polynucleotide is obtained that encodes for acetylcholinesterase in a targeted insect. The polynucleotide contains a catalytic core sequence. A recombinant DNA construct is formed by adding a fusion protein and a polyhistidine tag to the catalytic core sequence. The recombinant DNA construct can be further modified by adding known mutations for resistance to insecticides. A growth medium is transfected with the recombinant DNA construct. A polypeptide encoded by the recombinant DNA construct is separated from the growth medium to form a concentrate. The polyhistidine tag is removed from the concentrate. The concentrate is exchanged into a buffer to create a buffered concentrate. Crystals, suitable for X-ray crystallography are then grown with the buffered concentrate. |
| priorityDate | 2017-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.