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filingDate 2016-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f3ea75d5e888de4c24dea8a28af470e
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publicationDate 2020-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10619198-B2
titleOfInvention PCR primer linked to complementary nucleotide sequence or complementary nucleotide sequence including mis-matched nucleotides and method for amplifying nucleic acid using the same
abstract The present invention relates to a primer for PCR obtained by, directly or through inosine as a linker, linking a complementary nucleotide sequence or a complementary nucleotide sequence including a mis-matched nucleotide sequence to the 5′-terminal of a forward or reverse primer; and to a PCR method including a step of mixing a nucleic acid template in a PCR composition including the primer and then performing PCR on the mixture. The primer for PCR of the present invention includes a complementary nucleotide sequence or a mis-matched nucleotide sequence in a complementary nucleotide sequence, which is linked to the 5′-terminal thereof directly or via a linker, thereby lowering the sensitivity increase due to the increase in amplification products and reducing non-specifically occurring reactions in PCR.
priorityDate 2015-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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