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filingDate 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2019-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10450605-B2
titleOfInvention Pyrophosphorolytic sequencing
abstract A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.
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