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publicationDate 2019-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10426361-B2
titleOfInvention Quantification of absolute blood flow in tissue using fluorescence-mediated photoplethysmography
abstract A method and an apparatus for measuring a time-varying change in an amount of blood in a tissue include exciting a fluorescence agent in the blood, acquiring a time-varying light intensity signal during a pulsatile flow of the blood through the tissue volume, the pulsatile flow having a systolic and a diastolic phase resembling a conventional photoplethysmogram, and processing the acquired signal by applying a modified Beer-Lambert law to obtain the measurement of the time-varying change in the amount of blood in the tissue volume. The instantaneous molar concentration of the fluorescence agent is determined by utilizing a concentration-mediated change in a fluorescence emission spectrum of the fluorescence agent.
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