| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b79ea1c6c25451ae6f7c31cb9e750417
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2580cdb08c2c84de474963c93b779106 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-191
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-179 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6855
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2014-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2019-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b4b32dec5857e73ccb38bb2fd5c5e6a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5985d646fa512f69e581fcfc84604f4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9bc0469f61efcf4a5b9ac716b43541f4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dee42e0a145e4e6adc5e6e32bf6ef857
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e299f844e6f94d86078a11f56d8ae2e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_052ee68c58caae2783883a73e0e4b444 |
| publicationDate |
2019-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10344317-B2 |
| titleOfInvention |
Method and a sequence combination for producing nucleic acid fragments |
| abstract |
Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5′-end extension primer, wherein the single-stranded 5′-end extension primer comprises a sequencing platform adaptor sequence of a 5′ end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3′-end adaptor sequence to the 3′ end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends. |
| priorityDate |
2014-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |