http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10337059-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc2b03342511abc88f60c63f2acd96d0 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-119 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-301 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2015-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b37bd7e03bc851385254e6a6dfe7c73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_003f09a6de4921eb1dbdf157771528df |
| publicationDate | 2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-10337059-B2 |
| titleOfInvention | Enthalpy measurements for sequencing of a nucleic acid sample |
| abstract | The present method involves sequencing by synthesis in which a template strand having an attached primer is immobilized in a small volume reaction mixture. In one embodiment, the reaction mixture is in contact with a sensitive heat sensor, which detects the heat of reaction from incorporation of a complementary base (dNTP) in the presence of appropriate reagents (DNA polymerase, and polymerase reaction buffer). Alternatively, or in addition, a change in pH resulting from the incorporation of nucleotides in the DNA polymerase reaction is measured. A device is provided having delivery channels for appropriate reagents, including dNTPs, which may be delivered sequentially or in a mixture. Preferably, the dNTPs are added in a predetermined sequence, and the dNTP is incorporated or not depending on the template sequence. |
| priorityDate | 2006-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 373.