| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_15d42f684941d5b0617ca7764ebdff07 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70592
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-149 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1086
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-14 |
| filingDate |
2016-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2019-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21332eff31e0aa7281ed22e41720b635
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97518844d9289e0c092763a046c0bf7d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b953fd1755ded2751d3a4ff7d4d86fee |
| publicationDate |
2019-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10317329-B2 |
| titleOfInvention |
Early post-transfection isolation of cells (EPIC) for biologics production |
| abstract |
Provided herein are methods for selecting a population of cells expressing a target polypeptide. In some aspects, the disclosure provides methods for sorting and selecting populations of transfected host cells based on their early expression of a selectable polypeptide. In certain embodiments, the sorting is performed using fluorescence-activated cell sorting or magnetic-activated cell sorting based on the selectable polypeptide. Such selection methods can be further utilized to generate clonal populations of producer cells, e.g. for large-scale manufacturing of a target polypeptide of interest. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11635363-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11685943-B2 |
| priorityDate |
2015-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |