| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_de020b7dcbcaf745ca743fa95fa94853 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-447
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y404-01011
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-50 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate |
2015-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2019-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7081e3d59a3b8c3910521f51ed95fb25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_473223450b64e20f97c688af127871db
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74dba1b4c1159b4bfbff0c0f930f4599
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_789815cbb8f40497ec2383570591e1f2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af25ab561f7313698b988ebf3899c0be |
| publicationDate |
2019-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10308925-B2 |
| titleOfInvention |
Methionine lyase, encoding gene and biosynthetic method thereof |
| abstract |
Embodiment of the present invention discloses a kind of methionine lyase and its encoding gene and biosynthetic method. According to the present invention, the gene encoding methionine lyase as shown in SEQ ID No. 1 is separated from the genome of C. rosea . Embodiment of the present invention further provides an efficient biosynthetic method of methionine lyase, comprising: (1) cloning gene (shown in SEQ ID No.1) encoding methionine degradation enzyme into a yeast expression vector to construct recombinant yeast expression vector; (2) transforming the recombinant yeast expression vector into Saccharomyces cerevisiae to obtain expression strain; (3) inducing the expression strain to express the methionine lyase, collecting induced strains, purifying expressed recombinant methionine lyase. Purity of recombinant methionine lyase prepared according to the present invention is above 90%, and its efficiency of degradating methionine can reach 0.53±0.0030 μM MTL·h −1 ·mg protein −1 . |
| priorityDate |
2015-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |