http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10301606-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b0173e4934267fcf951f49a5a425d2dd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d7043b5431c4a24bc4fb5b1a6742dbcc |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 2018-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0cb9f644d7573148da0be9f4012d559 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db44fda1b93ae636374c09ebe54de2d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_381dc753e1e9422f48eb431c99356734 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_382d12d2d55cdffc105635ef5ef0c96c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3002d50550befb0dd2ff87db25a9f96d |
| publicationDate | 2019-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-10301606-B2 |
| titleOfInvention | DNA polymerase possessing continuous catalytic capacity and salt tolerance |
| abstract | A DNA polymerase, having an amino acid sequence represented by SEQ ID NO:2, or a derivative of the amino acid sequence by substitution, deletion, or addition of at least one amino acid residue. The DNA polymerase is a hybrid DNA polymerase prepared by inserting a thioredoxin binding domain (TBD) of bacteriophage T7 DNA polymerase into a DNA polymerase I (Sau) of Staphylococcus aureus . A method for preparing the DNA polymerase includes: 1) determining a corresponding position and a target substitution sequence in Sau protein for the TBD of the bacteriophage T7 DNA polymerase; 2) devising and synthesizing a primer according to a gene sequence of Sau and a sequence TBD published by GenBank; 3) cloning the Sau-TBD segment acquired in (2) to an expression vector pTrc99A to construct a recombinant vector pTrc99A-Sau-TBD; and 4) transforming Escherichia coli by the recombinant vector pTrc99A-Sau-TBD and inducing protein expression. |
| priorityDate | 2014-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 114.