| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55dd4da0d8f5bfa72abe0507617311d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-151
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-131
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-301
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-155 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate |
2018-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2019-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd67e932a93984cb2daf6b12fe8f26b7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6dc3cc5877590980b9c4d85a11e315f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbd401488e45b804b705ec988026ff4e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94d1d1bff337db5962fda19debfb29d0 |
| publicationDate |
2019-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10246706-B2 |
| titleOfInvention |
Methods and compositions for preventing concatemerization during template-switching |
| abstract |
Compositions and methods for performing a template-switching reaction are provided that may include reducing or eliminating concatemerization of the template-switching oligonucleotide (TSO). In some embodiments, the composition may comprise: a reverse transcriptase; a TSO that includes a recognition sequence for a site-specific double strand nucleic acid cleaving enzyme, wherein the TSO has at its 3′ end at least one nucleotide capable of hybridizing to at least one or more non-templated nucleotides added to a templated cDNA strand by the reverse transcriptase; and a site-specific double strand nucleic acid cleaving enzyme that cleaves the TSO at the recognition sequence. |
| priorityDate |
2016-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |