http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10208281-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a2bcaf91101a370a3d64e3190366357f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2537-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2537-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M25-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M25-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 2014-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_330d4ae879ba5ca53b9c81e49335e6ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f755c66e295ffb21c29a0d1252cefa9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63944968063f0046565edbfe05a61646 |
| publicationDate | 2019-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-10208281-B2 |
| titleOfInvention | Method for cultivating cells in adhesion culture by using a cell culture carrier in capsule form, and cell culture carrier therefor |
| abstract | The invention relates to a method for cultivating cells in adhesion culture, comprising at least the following steps: a) dissolving or suspending a cross-linkable, biocompatible material having adhesion points for cells in a cell culture medium; b) suspending cells in the cell culture medium, which contains the cross-linkable, biocompatible material, or in a medium that contains at least one component that is required for the cross-linking of the cross-linkable, biocompatible material; c) introducing the cell suspension into a medium in drops under conditions that initiate or permit the cross-linking of the biocompatible material, wherein either the cell suspension or the medium into which the cell suspension is introduced in drops contains the cross-linkable biocompatible material; d) forming stable, preferably porous capsules from cross-linked biocompatible material, which capsules contain incorporated adherent cells; e) proliferating the adherent cells in the capsules for a specified time period; f) breaking up the capsule material by means of a physical or chemical stimulus and releasing the cells as a cell suspension. In an especially preferred embodiment of the invention, the method is performed cyclically, in that the cells released in step f) are suspended anew in a cell culture medium that contains a cross-linkable, biocompatible material or in a medium that contains at least one component that is required for the cross-linking of the cross-linkable, biocompatible material and steps c)-f) are repeated at least once. |
| priorityDate | 2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.