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filingDate 2010-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10107820-B2
titleOfInvention Method of identifying peptides
abstract Methods of identifying polypeptides have been developed using a de novo sequencing technique. Methods use photodissociation and low-energy fragmentation and the spectra of peptide ions obtained therefrom, such as obtained by post-source decay (PSD), have been developed. The methods include photodissociation and the spectra therefrom obtainable from treating ions with predetermined wavelengths of radiation in the vacuum ultraviolet range of the electromagnetic spectrum. The confidence of amino acid assignments based on x-type ions is evaluated by observing complementary y-, v- and w-type ions that provide additional constraints to sequence identification.
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