Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50eb752940fa4aa36756a6bc9f6f3504 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2207-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-072 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2015-8527 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0387 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-7051 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-725 |
filingDate |
2015-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2018-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6f3ca937e60fa10b86d28df91ef5c0c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e09464a433b89ea9bf5043193b34ce0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67490e655088eb269019707ffe97f943 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7727bb04135a90f2bf6ff86c3f42642d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_052c655dd3bbe74281decd92a1900b45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8dfd4fd1f80b9a06d842af2667a6c03 |
publicationDate |
2018-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-10106820-B2 |
titleOfInvention |
Methods and compositions for modifying a targeted locus |
abstract |
Methods and compositions are provided for modifying one or more target loci in a cell. Such methods comprise providing a cell comprising a first polynucleotide encoding a first selection marker operably linked to a first promoter active in the cell, wherein the first polynucleotide further comprises a first recognition site for a first nuclease agent. A first nuclease agent is introduced into a cell, wherein the first nuclease agent induces a nick or double-strand break at the first recognition site. Further introduced into the cell is a first targeting vector comprising a first insert polynucleotide flanked by a first and a second homology arm that correspond to a first and a second target site located in sufficient proximity to the first recognition site. At least one cell is then identified comprising in its genome the first insert polynucleotide integrated at the target locus. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11576984-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11345932-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11697827-B2 |
priorityDate |
2014-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |