| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e41651508566f0385833e1ba14a21ffd |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2511-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-065
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0031
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0619
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0018
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-505 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-073
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-16 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate |
2013-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2018-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35398ff3195b83f8e00aa7e57b6ae0c8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69aa080b2bc68a03a76394ff8119a81d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_baf6779fd30e16317b0bc2e16dc80302
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c50122680633c4f861aad5b57d8abba2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcd66dc935ed5ff6d1a4bf7b2d61ad22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f63f394fe00ad06cd9717d980e471624
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2aa042da33c9ce65098dcb45bc817395 |
| publicationDate |
2018-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10066000-B2 |
| titleOfInvention |
High yield transient expression in mammalian cells using unique pairing of high density growth and transfection medium and expression enhancers |
| abstract |
High-Yield mammalian transient expression systems can include a cell culture media (particularly serum free, non animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11498949-B2 |
| priorityDate |
2012-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |