http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10059938-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9d0e1705700a059b200c774b76ce1234 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-307 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-119 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-137 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate | 2015-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7360066bc815397f3b330580a3f07b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27cfd8fc760fd3314f3df0eed48dd844 |
publicationDate | 2018-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-10059938-B2 |
titleOfInvention | Gene expression analysis |
abstract | The present invention is directed to methods and kits for gene analysis. The methods of the invention comprise the steps of providing nucleic acid; synthesis of a single-stranded DNA that is complementary to said nucleic acid molecule by contacting the nucleic acid with a DNA polymerase, a primer and a mixture of dNTPs under conditions that allow the generation of the DNA, wherein the primer comprises a target-complementary region and wherein the dNTP mixture comprises dATP, dGTP, dCTP, dTTP and dUTP; cleaving the DNA 5′ to dU sites by (i) contacting the DNA with an uracil deglycosylase to generate a basic sites at positions of dUTP incorporation in the DNA; and (ii) contacting the DNA with an apurinic/apyrimidinic (AP) endonuclease; contacting the DNA comprising at its 5′-end the nucleotide sequence of the primer with a ssDNA ligase to circularize the DNA; and sequencing the circularized cDNA. The kits comprise the components necessary to perform the methods of the invention. |
priorityDate | 2014-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 251.