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filingDate 2013-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2018-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10031127-B2
titleOfInvention Method for separating aggregates of malignant cells and aggregates from stromal cells of a malignant tumour tissue sample
abstract An ex vivo malignant tumor tissue sample is cleaned, comminuted, suspended in culture medium, subjected to a treatment with collagenase and the collagenase decomposition product is centrifuged. The pellet obtained is re-suspended in culture medium by an absorption instrument, is absorbed and returned several times and transferred to a culture vessel. The re-suspension thus obtained is analyzed under a microscope with phase optics for cell aggregates, which, due to the phenotypic appearance thereof, are identifiable as an aggregate of malignant cells or as an aggregate of endothelial cells or as an aggregate of fibroblasts, and the identified cell aggregates are separated.
priorityDate 2012-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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