http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10030260-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18d92c46f60af8af24e262b67381d7eb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-952 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-04 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| filingDate | 2013-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13257a094c27d0ab61ed097fb93236f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e402ebd17a8d1787d492ae705f18aa7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44bbe0b59a836fdc488d2dae3a292cd1 |
| publicationDate | 2018-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-10030260-B2 |
| titleOfInvention | Inducible reconstitution and real-time quantitative kinetic system for the study of intramembrane enzymes |
| abstract | The present invention relates to the field of enzymes. More specifically, the present invention provides an inducible reconstitution and real-time quantitative kinetic system for the study of intramembrane enzymes. In a specific embodiment, a method of screening for modulators of an intramembrane protease comprises the steps of (a) contacting in a mixture the protease and a substrate with a lipid under acidic or basic conditions to form a membrane comprising the lipid bilayer, protease and the substrate; (b) contacting a test agent with the membrane mixture; (c) adjusting the pH to physiological conditions; (d) assaying substrate cleavage by the protease; and (e) comparing the assayed substrate cleavage to a reference that does not include the test agent, wherein an increase or a decrease of substrate cleavage by the protease relative to the reference identifies the test agent as a modulator of the intramembrane protease. |
| priorityDate | 2012-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 402.