| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f535660b0a0e565cf1ec41411bae18e5 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6881 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6881
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate |
2013-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2018-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04ab03629502c0191d426ff3d0a36f00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa811f35beb07dbd8ae80d5e389aa239
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_910ff5f17c2c38353888402a101f4231
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbbf6aba9d71acdda7e9dea0f3df7eac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_38b772d1c38e6cadbf72f1964ffb1910
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46d29bd72c0943aad3047a2fdc28040c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_990bd5fef48f137bdf201a438324c8fe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19753e7f8bea6bf3ee8281f19e1864d6 |
| publicationDate |
2018-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10017824-B2 |
| titleOfInvention |
Method for determining nucleic acid composition of nucleic acid mixture |
| abstract |
The present invention provides a method for determining the nucleic acid composition in a total nucleic acid mixture comprising a first nucleic acid and a second nucleic acid. The method comprises: 1) treating the total nucleic acid mixture with a bisulfate, to convert the non-methylated cytosine in the total nucleic acid mixture into uracil, and obtain a converted total nucleic acid mixture; 2) subjecting the converted total nucleic acid mixture to multiplexed fluorescent quantitative PCR using a first set of amplification primers and a second set of amplification primers; and 3) based on the ratio R of the methylated amplification product to the non-methylated amplification product of the predetermined nucleic acid fragment, a methylation proportion M 1 of the predetermined nucleic acid fragment in the first nucleic acid, and a methylation proportion M 2 of the predetermined nucleic acid fragment in the second nucleic acid, determining the nucleic acid composition in the total nucleic acid mixture. |
| priorityDate |
2013-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |