http://rdf.ncbi.nlm.nih.gov/pubchem/patent/UA-116765-U
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61B10-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2016-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | UA-116765-U |
| titleOfInvention | METHOD OF EARLY FORECAST OF RISK OF DEVELOPMENT OF Uterine BODY CANCER IN WOMEN WITH HYPERPROLIFERATIVE ENDOMETRY PROCESSES |
| abstract | A method for early prediction of the risk of uterine cancer in women with endometrial hyperproliferative processes involves genetic examination of endometrial tissue. Use the semi-quantitative method of PCR PC, for which DNA is extracted from tissue samples using the reagent DNeasy Blood & Tissue Kit (Qiagen), while bisulfite DNA processing is carried out using the kit EpiTect Bisulfite Kit (Qiagen) according to the protocol, the amount of DNA for the processing of bisulfite in all samples adjusted to 1 µg / ml, carried out DNA amplification using the Hot Start DNA Polymeraza Kit program: 95 ° C - 15 minutes; 95 ° C - 30 s, 50 ° C for unmethylated DNA and 60 ° C for methylated DNA, 72 ° C for 30 s, 39 cycles; 72 ° C for 10 minutes, then conduct the PCR electrophoresis of the products in 2% agarose, then stained with ethidium bromide for 20 minutes, analyze the relative content of methylated and unmethylated DNA using Quantity One ID Analysis Software (BIO-RAD, USA) on the device VersaDoc MP 4000 System (BIO-RAD, USA) according to the formula M (%) = A × 100 / A + B, where: A is the peak area of methylated DNA, B is the peak area of unmethylated DNA and at the values of the content of methylated DNA of the gene SFRP2 above 25% predict the risk of uterine cancer in patients with endometrial hyperproliferative processes. |
| priorityDate | 2016-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.