http://rdf.ncbi.nlm.nih.gov/pubchem/patent/UA-114767-U
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
filingDate | 2017-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | UA-114767-U |
titleOfInvention | METHOD OF OBTAINING CULTURES OF MESENCHIMAL-STROMAL CELLS FOR FURTHER CULTIVATION FOR THERAPEUTIC APPLICATION |
abstract | A method of obtaining a culture of mesenchymal-stromal cells for their further cultivation for therapeutic use involves the preparation of a human umbilical cord with obtaining the crushed umbilical cord tissue. Isolation of mesenchymal-stromal fraction of cells from the umbilical cord by incubation of the umbilical cord in serum-free DMEM in the presence of 0.1% collagenase type A followed by resuspension of cell pellet in DMEM / F12 growth medium containing 10% neonatal calf serum, 2 mm l of glutamine, 1% of essential amino acids and 1% of penicillin and streptomycin. |
priorityDate | 2017-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 15.