| abstract |
The present application provides a method for generating chloroplast gene modified plant cells, comprising: constructing a pair of nuclear gene modifying vectors; transforming the pair of nuclear gene modifying vectors into plant cells vian Agrobacterium ; and selecting and culturing the plant cells. The nuclear gene modifying vectors comprises a promoter, a recombinant gene conferred the chloroplast transit peptide and TALEN protein domain, a terminator as well as right border and left border of T-DNA regions. Thus, by using nuclear transformation to mutate chloroplast gene, the problems such as low transformation efficiency, long selection time, labor-intensive and high cost encountering in chloroplast transformation would be avoided. |