| abstract |
A method for producing a virus-like particle (VLP) in a plant is provided. The method includes introducing a first nucleic acid into a plant or plant part. The first nucleic acid comprises a first regulatory region that is active in a plant and is operably linked to a nucleotide sequence encoding one or more rotavirus structural proteins (such as, but not limited to, rotavirus protein VP2). . The nucleotide sequence may also include one or more amplification elements and / or compartment targeting sequences. The second nucleic acid can be introduced into a plant or a part of a plant. The second nucleic acid comprises a second regulatory region that is active in a plant and is operably linked to a nucleotide sequence encoding one or more rotavirus structural proteins (such as, but not limited to, rotavirus protein VP6). Optionally, the third nucleic acid and / or the fourth nucleic acid may be introduced into a plant or a part of a plant. The third nucleic acid comprises a third regulatory region that is active in a plant and is operably linked to a nucleotide sequence encoding one or more rotavirus structural proteins (such as, but not limited to, rotavirus protein VP4). The fourth nucleic acid comprises a fourth regulatory region that is active in a plant and is operably linked to a nucleotide sequence encoding one or more rotavirus structural proteins (such as, but not limited to, rotavirus protein VP7). The plant or plant part is cultivated while allowing expression of the nucleic acid, thereby producing VLP. |