http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-I278518-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_15914dfae1086193a5b9c15bb52902fb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0653 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3895 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate | 2000-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2007-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1b34cce03319e99a90491dd7d18e4b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e790af2f912f3ba0e5fe3d8f8591f073 |
| publicationDate | 2007-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TW-I278518-B |
| titleOfInvention | Methods and compositions for the differentiation of human preadipocytes into adipocytes |
| abstract | The present invention provides methods and compositions for the consistent and quantitative differentiation of human preadipocytes isolates from adipose tissue into adipocytes bearing biochemical, genetic, and physiological characteristics similar to that observed in isolated primary adipocytes. The methods of the invention comprise incubating isolated human preadipocytes, plated at least about 25,000 cells/cm<2>, in a medium containing, glucose, a cyclic AMP inducer such as isobutylmethylxanthine or forskolin, a glucocorticoid or glucocorticoid analogue, insulin or an insulin analogue and a PPARgamma agonist or a RXR agonist. Also provided are methods for preparing three dimensional biomatrices containing adipocytes differentiated by the methods of the invention. The compositions of the invention include human adipocytes differentiated by the methods of the invention, three-dimensional matrices of adipocytes, and transfected adipocytes. The methods and compositions have use in the drug discovery of compounds having relevance to the disease states of diabetes, obesity, and cardiovascular disease and in the studies of these diseases, and in the grafting of fat tissue. |
| priorityDate | 1999-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 402.